Anti-Inflammatory Effects of Fermented Bark of Acanthopanax sessiliflorus and Its Isolated Compounds on Lipopolysaccharide-Treated RAW 264.7 Macrophage Cells.
Min Ji KimHye Soo WangMin-Won LeePublished in: Evidence-based complementary and alternative medicine : eCAM (2020)
The fermentation was carried out on the bark of Acanthopanax sessiliflorus (AS). Acanthopanax species have been used in traditional medicine as tonics, sedatives, and antispasmodics. An activity-guided isolation of the fermented bark of A. sessiliflorus (FAS) yielded several phytochemicals: acanthoside D (1), acanthoside B (2), daucosterol (3), protocatechuic acid (4), chlorogenic acid methyl ester (5), ciwujiatone (6), syringaresinol (7), farnesol (8), 3,4-dicaffeoylquinic acid (9), and falcarindiol (10). HPLC analysis showed that content of lignan glycoside (1) was decreased and 4 and 7 were increased after fermentation. Anti-inflammatory activities on FAS showed the decrease of nitric oxide (NO) production, and inhibitory activities of iNOS and COX-2, proinflammatory cytokines (IL-6 and tumor necrosis factor-α), and collagenase. The aglycone, syringaresinol (7), which was increased through fermentation showed enhanced activity than 1. Thus, FAS may have the potential to treat inflammatory disorders, such as arthritis.
Keyphrases
- lactic acid
- nitric oxide
- saccharomyces cerevisiae
- rheumatoid arthritis
- anti inflammatory
- induced apoptosis
- ms ms
- inflammatory response
- nitric oxide synthase
- toll like receptor
- adipose tissue
- mass spectrometry
- hydrogen peroxide
- candida albicans
- immune response
- newly diagnosed
- high performance liquid chromatography
- cell death
- high resolution
- climate change
- pi k akt
- tandem mass spectrometry