A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.
Huagang HeZhaozhao ChenRenchun FanJie ZhangShanying ZhuJiale WangQianyuan ZhangAnli GaoShuangjun GongLu ZhangYanan LiYitong ZhaoSimon G KrattingerQian-Hua ShenHongjie LiYajun WangPublished in: Nature communications (2024)
Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders the cloning of the introgressed genes. Here, we have cloned the powdery mildew resistance gene Pm13, which is introgressed into wheat from Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of Pm13 is validated through transient and stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves and wheat protoplasts reveal that the fragment Brace-Kinase 122-476 of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three α-helices in the brace region close to the N-terminus of the kinase domain.
Keyphrases
- protein kinase
- tyrosine kinase
- genome wide
- radiation induced
- cell death
- single cell
- air pollution
- particulate matter
- mental health
- radiation therapy
- gene expression
- poor prognosis
- heavy metals
- rna seq
- genome wide identification
- dna methylation
- dna damage
- mass spectrometry
- high throughput
- dna repair
- ionic liquid
- signaling pathway
- brain injury
- drug induced
- blood brain barrier
- water soluble
- cell fate