Diagnostic performance and comparison of ultrasensitive and conventional rapid diagnostic test, thick blood smear and quantitative PCR for detection of low-density Plasmodium falciparum infections during a controlled human malaria infection study in Equatorial Guinea.
Maximillian G MpinaThomas C StablerTobias SchindlerJose RasoAnna DealLudmila Acuche PupuElizabeth NyakarunguMaria Del Carmen Ovono DavisVicente UrbanoAli MtoroAli HamadMaria Silvia A LopezBeltran PasialoMarta Alene Owono EyangMatilde Riloha RivasCarlos Cortes FallaGuillermo A GarcíaJuan Carlos MomoRaul ChuquiyauriElizabeth SaverinoL W Preston ChurchB Kim Lee SimBonifacio ManguireMarcel TannerCarl MaasSalim AbdullaPeter F BillingsleyStephen L HoffmanSaid JongoThomas L RichieClaudia A DaubenbergerPublished in: Malaria journal (2022)
TBS and uRDT had similar sensitivities, both were more sensitive than cRDT, and neither matched qPCR for detecting low density parasitaemia. uRDT could be considered an alternative to TBS in selected applications, such as CHMI or field diagnosis, where qualitative, dichotomous results for malaria infection might be sufficient.