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Cost-effective DNA methylation profiling by FML-seq.

Joseph W FoleyShirley X ZhuRobert B West
Published in: bioRxiv : the preprint server for biology (2023)
Current methods for profiling DNA methylation require costly reagents, sequencing, or labor time. We introduce FML-seq, a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.
Keyphrases
  • single cell
  • dna methylation
  • genome wide
  • rna seq
  • high throughput
  • gene expression
  • endothelial cells
  • randomized controlled trial
  • copy number
  • quality improvement