Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE).

Kristian Alsbjerg SkipperAnne Kruse HollensenMichael N AntoniouJacob Giehm Mikkelsen

Published in: BMC biotechnology (2019)

Our findings contribute to the understanding of the mechanism of HNRPA2B1-CBX3 UCOE-based transgene protection and support the use of a correctly oriented core fragment of this UCOE for DNA transposon vector-based production of recombinant proteins in CHO-K1 cells.

Keyphrases

cell free
circulating tumor
induced apoptosis
single molecule
poor prognosis
cell cycle arrest
gene expression
dna damage
transcription factor
genome wide
circulating tumor cells
long non coding rna
cell proliferation
pi k akt