Fluorescent CXCR4 targeting peptide as alternative for antibody staining in Ewing sarcoma.
Laurens G L SandTessa BuckleFijs W B van LeeuwenWillem E CorverAlwine B KruisselbrinkAart G JochemsenPancras C W HogendoornKároly SzuhaiPublished in: BMC cancer (2017)
The fluorescently labeled CXCR4 targeting peptide-based method provides a reliable alternative to antibody staining to study the CXCR4 membrane expression in live cells using either flow cytometry or live cell fluorescence microscopy. The fluorescently tagged CXCR4 targeting peptide could enable in vivo detection of CXCR4 expression in Ewing sarcoma which may help to stratify cases for anti-CXCR4 therapy.
Keyphrases
- flow cytometry
- cell migration
- poor prognosis
- cancer therapy
- induced apoptosis
- single molecule
- label free
- high resolution
- signaling pathway
- mesenchymal stem cells
- oxidative stress
- bone marrow
- endoplasmic reticulum stress
- drug delivery
- optical coherence tomography
- cell cycle arrest
- living cells
- cell death
- pet imaging
- high speed
- real time pcr