Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults.
Nachon RaethongMassalin NakphaichitNarissara SuratannonWitida SathitkowitchaiWanlapa WeerapakornSuttipun KeawsompongWanwipa VongsangnakPublished in: Genes (2021)
The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country.
Keyphrases
- endothelial cells
- human health
- single cell
- risk assessment
- genome wide
- induced pluripotent stem cells
- healthcare
- public health
- pluripotent stem cells
- copy number
- electronic health record
- climate change
- genome wide identification
- dna methylation
- gene expression
- microbial community
- health information
- deep learning
- network analysis
- cell wall
- health promotion