Uniform Gold Nanostructure Formation via Weakly Adsorbed Gold Films and Thermal Annealing for Reliable Localized Surface Plasmon Resonance-Based Detection of DNase-I.
Joon-Ha ParkChi-Pin James WangHye-Jin LeeKyung Soo HongJung Hong AhnYeon-Woo ChoJeong-Hyeon LeeHee Seung SeoWooram ParkSe-Na KimChun Gwon ParkWonhwa LeeTae-Hyung KimPublished in: Small (Weinheim an der Bergstrasse, Germany) (2023)
Deoxyribonuclease-I (DNase-I), a representative endonuclease, is an important biomarker for the diagnosis of infectious diseases and cancer progression. However, enzymatic activity decreases rapidly ex vivo, which highlights the need for precise on-site detection of DNase-I. Here, a localized surface plasmon resonance (LSPR) biosensor that enables the simple and rapid detection of DNase-I is reported. Moreover, a novel technique named electrochemical deposition and mild thermal annealing (EDMIT) is applied to overcome signal variations. By taking advantage of the low adhesion of gold clusters on indium tin oxide substrates, both the uniformity and sphericity of gold nanoparticles are increased under mild thermal annealing conditions via coalescence and Ostwald ripening. This ultimately results in an approximately 15-fold decrease in LSPR signal variations. The linear range of the fabricated sensor is 20-1000 ng mL -1 with a limit of detection (LOD) of 127.25 pg mL -1 , as demonstrated by spectral absorbance analyses. The fabricated LSPR sensor stably measured DNase-I concentrations from samples collected from both an inflammatory bowel disease (IBD) mouse model, as well as human patients with severe COVID-19 symptoms. Therefore, the proposed LSPR sensor fabricated via the EDMIT method can be used for early diagnosis of other infectious diseases.
Keyphrases
- infectious diseases
- gold nanoparticles
- label free
- mouse model
- loop mediated isothermal amplification
- sars cov
- real time pcr
- endothelial cells
- coronavirus disease
- magnetic resonance imaging
- cross sectional
- reduced graphene oxide
- hydrogen peroxide
- escherichia coli
- sensitive detection
- papillary thyroid
- optical coherence tomography
- early onset
- computed tomography
- induced pluripotent stem cells
- ulcerative colitis
- quantum dots
- lymph node metastasis
- cystic fibrosis
- respiratory syndrome coronavirus