Application of Recombinase Polymerase Amplification with CRISPR/Cas12a and Multienzyme lsothermal Rapid Amplification with lateral flow dipstick assay for Bactrocera correcta.

Our research findings demonstrate that both the RPA-CRISPR/Cas12a and MIRA-LFD methods for B. correcta detection was accurate and rapid (within 30 min and 10 min, respectively), at 37°C. Our methods do not rely on expensive equipment, thus possess high practical value, providing improved identification solutions for port quarantine pests and field applications. This article is protected by copyright. All rights reserved.