Quantification of carbamylated albumin in serum based on capillary electrophoresis.
Sigurd DelangheAlena MoermanAnneleen PletinckEva SchepersGriet GlorieuxWim Van BiesenJoris Richard DelangheMarijn M SpeeckaertPublished in: Electrophoresis (2017)
Protein carbamylation, a nonenzymatic posttranslational modification promoted during uremia, is linked to a poor prognosis. In the present study, carbamylation of serum albumin was assayed using the symmetry factor on a capillary electrophoresis instrument (Helena V8). The symmetry factor has been defined as the distance from the center line of the peak to the back slope, divided by the distance from the center line of the peak to the front slope, with all measurements made at 10% of the maximum peak height. Serum albumin, creatinine, and urea concentrations were assayed using routine methods, whereas uremic toxins were determined using HPLC. In vitro carbamylation induced a marked albumin peak asymmetry. Reference values for the albumin symmetry factor were 0.69-0.92. In kidney patients, albumin peak asymmetry corresponded to the chronic kidney disease stage (p < 0.0001). The symmetry factor correlated well with serum urea (r = -0.5595, p < 0.0001) and creatinine (r = -0.5986, p < 0.0001) concentrations. Several protein-bound uremic toxins showed a significant negative correlation with the symmetry factor. Morphology of the albumin fraction was not affected by presence of glycated albumin and protein-bound antibiotics. In conclusion, the presented method provides a simple, practical way for monitoring protein carbamylation.
Keyphrases
- capillary electrophoresis
- poor prognosis
- chronic kidney disease
- end stage renal disease
- mass spectrometry
- protein protein
- amino acid
- long non coding rna
- ejection fraction
- ms ms
- newly diagnosed
- peritoneal dialysis
- binding protein
- metabolic syndrome
- small molecule
- uric acid
- patient reported outcomes
- simultaneous determination
- patient reported