Strain and rupture of HIV-1 capsids during uncoating.
Alvin YuElizabeth M Y LeeJohn A G BriggsBarbie K Ganser-PornillosOwen PornillosGregory A VothPublished in: Proceedings of the National Academy of Sciences of the United States of America (2022)
SignificanceThe mature capsids of HIV-1 are transiently stable complexes that self-assemble around the viral genome during maturation, and uncoat to release preintegration complexes that archive a double-stranded DNA copy of the virus in the host cell genome. However, a detailed view of how HIV cores rupture remains lacking. Here, we elucidate the physical properties involved in capsid rupture using a combination of large-scale all-atom molecular dynamics simulations and cryo-electron tomography. We find that intrinsic strain on the capsid forms highly correlated patterns along the capsid surface, along which cracks propagate. Capsid rigidity also increases with high strain. Our findings provide fundamental insight into viral capsid uncoating.
Keyphrases
- antiretroviral therapy
- hiv positive
- molecular dynamics simulations
- hiv infected
- hiv testing
- human immunodeficiency virus
- hepatitis c virus
- hiv aids
- men who have sex with men
- sars cov
- south africa
- single cell
- cell therapy
- mental health
- molecular dynamics
- molecular docking
- circulating tumor
- binding protein
- mass spectrometry