ONE ASSAY TO TEST THEM ALL: COMPARING MULTIPLEX ASSAYS FOR EXPANSION OF RESPIRATORY VIRUS SURVEILLANCE.
Narjis BoukliClaude FlamandKim Lay CheaLeangyi HengSeangmai KeoKimhoung SourSophea InPanha ChhimBunthea ChhorLomor KruyJelena D M FeenstraManoj GandhiObiageli OkaforCamilla UleklievHeidi AuerswaldViseth Srey HormErik Albert KarlssonPublished in: medRxiv : the preprint server for health sciences (2023)
Viral respiratory infections represent a major burden globally, weighed down by the COVID-19 pandemic, and threatened by spillover of novel zoonotic influenza viruses. Since respiratory infections share clinical presentations, identification of the causing agent for patient care and public health measures requires laboratory testing for several pathogens, including potential zoonotic spillovers. Simultaneous detection of SARS-CoV-2, influenza, and RSV in a single RT-PCR accelerates time from sampling to diagnosis, preserve consumables, and streamline human resources to respond to other endemic or emerging pathogens. Multiplex assays have the potential to sustain and even expand surveillance systems, can utilize capacity/capability developed during the COVID-19 pandemic worldwide, thereby strengthening epidemic/pandemic preparedness, prevention, and response.
Keyphrases
- public health
- sars cov
- high throughput
- real time pcr
- respiratory tract
- gram negative
- respiratory syndrome coronavirus
- endothelial cells
- global health
- single cell
- coronavirus disease
- human health
- induced pluripotent stem cells
- loop mediated isothermal amplification
- respiratory syncytial virus
- label free
- risk factors
- infectious diseases
- genetic diversity