Effects of progesterone nanoparticles on the sperm capacitation and acrosome reaction in asthenozoospermia men.
Khadijeh BaranizadehMohammad Mehdi MahboobianIraj AmiriHeidar TavilaniGholamreza ShafieePublished in: Andrologia (2021)
Low motility is one of the causes of male infertility. In this study, the effects of progesterone solid lipid nanoparticles (SLNs) on sperm capacitation, acrosome reaction, oxidative stress and expression of SPACA1 and MAPK way genes were investigated. Progesterone SLNs were synthesized using the solvent emulsification evaporation method. Twenty asthenozoospermia samples were selected, and sperm and acrosome membrane integrity, acrosome reaction, sperm motility, viability, total antioxidant capacity (TAC), total oxidative status tests and PKA, PTK, P38MAPK and SPACA1 gene expressions were assessed. The synthesized nanoparticles were prepared with the size (187.6 nm), PDI (0.184), EE (85.82%), LP (3.43%) and ZP (-23.5mV). Progesterone SLNs increased sperm and acrosome membrane integrity and TAC (p < .05). Also, the expression of P38MAPK, PKA, PTK, and SPACA1 genes in this group showed a significant increase (p < .001). Progesterone SLNs increased acrosome reaction, sperm capacitation and TAC. Also, it increased the expression of PTK PKA, SPACA1 and P38MAPK genes.
Keyphrases
- poor prognosis
- oxidative stress
- genome wide
- estrogen receptor
- genome wide identification
- binding protein
- dna damage
- biofilm formation
- type diabetes
- signaling pathway
- metabolic syndrome
- insulin resistance
- staphylococcus aureus
- copy number
- cell proliferation
- escherichia coli
- high resolution
- heat shock protein
- skeletal muscle
- polycystic ovary syndrome
- heat shock
- atomic force microscopy
- candida albicans