Assessment of antioxidant and antidiabetic properties of Agaricus blazei Murill extracts.
Qi WeiYishu ZhanBingzhi ChenBaogui XieTing FangSadhana RavishankarYuji JiangPublished in: Food science & nutrition (2019)
Agaricus blazei Murill (ABM), a medicinal mushroom, has beneficial effects on various human metabolic diseases. The objective of this research was to evaluate the antioxidant and antidiabetic properties of ABM extracts (ethanol extract and ethyl acetate extract). The antioxidant activities of ABM ethanol extract (EE) and ethyl acetate extract (EA) were analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radical scavenging assays and the reducing power using K3Fe(CN)6 in vitro. Moreover, the effects of EE and EA on α-glucosidase inhibitory activity and improving glucose uptake by HepG2 cells were investigated in vitro. The EA showed stronger antioxidant activity, as well as inhibition of α-glucosidase, compared to EE. The analysis of glucose uptake by HepG2 cells showed that EA had significant glucose-lowering activity and exhibited no difference compared to metformin. The results suggest that ABM extracts could improve the glucose uptake by HepG2 cells and thereby alleviate postprandial hyperglycemia. This investigation provides a strong rationale for further studies on the application of ABM to control type 2 diabetes.
Keyphrases
- oxidative stress
- anti inflammatory
- blood glucose
- type diabetes
- ionic liquid
- molecular docking
- endothelial cells
- glycemic control
- diabetic rats
- cardiovascular disease
- clinical trial
- squamous cell carcinoma
- metabolic syndrome
- mass spectrometry
- induced pluripotent stem cells
- high resolution
- single cell
- molecular dynamics simulations
- atomic force microscopy
- single molecule
- clinical evaluation