MoS 2 -Based Sensor Array for Accurate Identification of Cancer Cells with Ensemble-Modified Aptamers.
Ying XiangJingjing LiuJing ChenMingshu XiaoHao PeiLi LiPublished in: ACS applied materials & interfaces (2024)
In this work, we present an array-based chemical nose sensor that utilizes a set of ensemble-modified aptamer (EMAmer) probes to sense subtle physicochemical changes on the cell surface for cancer cell identification. The EMAmer probes are engineered by domain-selective incorporation of different types and/or copies of positively charged functional groups into DNA scaffolds, and their differential interactions with cancer cells can be transduced through competitive adsorption of fluorophore-labeled EMAmer probes loaded on MoS 2 nanosheets. We demonstrate that this MoS 2 -EMAmer-based sensor array enables rapid and effective discrimination among six types of cancer cells and their mixtures with a concentration of 10 4 cells within 60 min, achieving a 94.4% accuracy in identifying blinded unknown cell samples. The established MoS 2 -EMAmer sensing platform is anticipated to show significant promise in the advancement of cancer diagnostics.
Keyphrases
- quantum dots
- reduced graphene oxide
- single molecule
- high throughput
- room temperature
- small molecule
- nucleic acid
- high resolution
- transition metal
- cell surface
- visible light
- living cells
- sensitive detection
- fluorescence imaging
- gold nanoparticles
- highly efficient
- fluorescent probe
- induced apoptosis
- ionic liquid
- drug delivery
- single cell
- convolutional neural network
- high density
- cell cycle arrest
- bioinformatics analysis
- computed tomography
- neural network
- squamous cell carcinoma
- randomized controlled trial
- cell death
- squamous cell
- circulating tumor
- tissue engineering
- metal organic framework
- machine learning
- mass spectrometry
- loop mediated isothermal amplification
- wound healing
- big data
- mesenchymal stem cells