Biochemical and Structural Insights into the Winged Helix Domain of P150, the Largest Subunit of the Chromatin Assembly Factor 1.
Joëlle AyoubMartina BuonannoAnna Di FioreGiuseppina De SimoneSimona Maria MontiPublished in: International journal of molecular sciences (2022)
The Chromatin Assembly Factor 1 is a heterotrimeric complex responsible for the nucleosome assembly during DNA replication and DNA repair. In humans, the largest subunit P150 is the major actor of this process. It has been recently considered as a tumor-associated protein due to its overexpression in many malignancies. Structural and functional studies targeting P150 are still limited and only scarce information about this subunit is currently available. Literature data and bioinformatics analysis assisted the identification of a stable DNA binding domain, encompassing residues from 721 to 860 of P150 within the full-length protein. This domain was recombinantly produced and in vitro investigated. An acidic region modulating its DNA binding ability was also identified and characterized. Results showed similarities and differences between the P150 and its yeast homologue, namely Cac-1, suggesting that, although sharing a common biological function, the two proteins may also possess different features.
Keyphrases
- high resolution
- dna binding
- transcription factor
- dna repair
- bioinformatics analysis
- dna damage
- systematic review
- gene expression
- health information
- protein kinase
- genome wide
- dna damage response
- cell proliferation
- social media
- signaling pathway
- electronic health record
- atomic force microscopy
- ionic liquid
- big data
- oxidative stress
- healthcare
- machine learning
- amino acid
- case control
- single molecule