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High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting.

Xu-Dong ZhuXiang ShiShu-Wen WangJu ChuWei-Hong ZhuBang-Ce YePeng ZuoYong-Hong Wang
Published in: RSC advances (2019)
A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans . In this system, water-in-oil-in-water (W/O/W) droplets, which were ∼12 pL in volume were used as picoliter-reactors for lactic acid fermentation. A fluorescent sensor was developed and used for monitoring pH which indicated the production of lactic acid. After fermentation, fluorescence activated cell sorting was performed with high sensitivity and speed. Using this microfluidic high-throughput screening system, we found a mutant with a yield of 76 g L -1 lactic acid which was 52% higher than its parent strain with a screening throughput exceeding 10 6 clones per h.
Keyphrases
  • lactic acid
  • single cell
  • high throughput
  • flow cytometry
  • circulating tumor cells
  • single molecule
  • label free
  • cell therapy
  • quantum dots
  • energy transfer
  • bone marrow
  • wild type