Mesenchymal Stem Cells from Familial Alzheimer's Patients Express MicroRNA Differently.
Lory J Rochín-HernándezLory S Rochín-HernándezMayte L Padilla-CristernaAndrea Duarte-GarcíaMiguel A Jiménez-AcostaMaría P Figueroa-CoronaMarco Antonio Meraz-RiosPublished in: International journal of molecular sciences (2024)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the predominant form of dementia globally. No reliable diagnostic, predictive techniques, or curative interventions are available. MicroRNAs (miRNAs) are vital to controlling gene expression, making them valuable biomarkers for diagnosis and prognosis. This study examines the transcriptome of olfactory ecto-mesenchymal stem cells (MSCs) derived from individuals with the PSEN1(A431E) mutation (Jalisco mutation). The aim is to determine whether this mutation affects the transcriptome and expression profile of miRNAs and their target genes at different stages of asymptomatic, presymptomatic, and symptomatic conditions. Expression microarrays compare the MSCs from mutation carriers with those from healthy donors. The results indicate a distinct variation in the expression of miRNAs and mRNAs among different symptomatologic groups and between individuals with the mutation. Using bioinformatics tools allows us to identify target genes for miRNAs, which in turn affect various biological processes and pathways. These include the cell cycle, senescence, transcription, and pathways involved in regulating the pluripotency of stem cells. These processes are closely linked to inter- and intracellular communication, vital for cellular functioning. These findings can enhance our comprehension and monitoring of the disease's physiological processes, identify new disorder indicators, and develop innovative treatments and diagnostic tools for preventing or treating AD.
Keyphrases
- mesenchymal stem cells
- gene expression
- cell cycle
- stem cells
- umbilical cord
- genome wide
- poor prognosis
- dna methylation
- end stage renal disease
- early onset
- rna seq
- newly diagnosed
- prognostic factors
- chronic kidney disease
- cell therapy
- single cell
- multiple sclerosis
- bone marrow
- ejection fraction
- cognitive decline
- physical activity
- cognitive impairment
- binding protein
- long non coding rna
- rectal cancer
- sensitive detection
- patient reported
- quantum dots
- living cells