Near-infrared enhances antiangiogenic potentiality of quinacrine-gold hybrid nanoparticles in breast cancer stem cells via deregulation of HSP-70/TGF-β.
Somya Ranjan DashBiswajit DasChinmay DasSaptarshi SinhaSubarno PaulRajalaxmi PradhanChanakya Nath KunduPublished in: Nanomedicine (London, England) (2023)
Aim: This study aimed to explore the antiangiogenic mechanism of quinacrine-gold hybrid nanoparticle (QAuNP) and near-infrared (NIR) radiation in patient-derived primary breast cancer stem cells. Materials & methods: Various cell-based in ovo angiogenesis and in vivo patient-derived xenograft mouse systems were used as models for the study. Results: The experimental results showed that QAuNP + NIR treatment deregulated the HSP-70/TGF-β physical interaction in primary breast cancer stem cells. Reduced TGF-β secretion in the tumor microenvironment inhibited angiogenesis activation in endothelial cells by deregulating the TGF-β-mediated PI3K/AKT/mTOR cascade. Conclusion: This study revealed that QAuNP + NIR irradiation downregulated HSP-70 expression, inhibited the HSP-70/TGF-β interaction, reduced the secretion of TGF-β in the tumor microenvironment and ultimately inhibited TGF-β-mediated angiogenesis.
Keyphrases
- cancer stem cells
- transforming growth factor
- endothelial cells
- heat shock protein
- photodynamic therapy
- heat shock
- heat stress
- epithelial mesenchymal transition
- vascular endothelial growth factor
- poor prognosis
- physical activity
- oxidative stress
- fluorescence imaging
- drug release
- cell therapy
- signaling pathway
- long non coding rna
- silver nanoparticles