Avidity and Cell Uptake of Integrin-Targeting Polypeptide Micelles is Strongly Shape-Dependent.
Michael J DzurickySinan XiongPatrick WeberAshutosh ChilkotiPublished in: Nano letters (2019)
We describe a genetically encoded micelle for targeted delivery consisting of a diblock polypeptide with segments derived from repetitive protein motifs inspired by Drosophila melanogaster Rec-1 resilin and human tropoelastin with a C-terminal fusion of an integrin-targeting fibronectin type III domain. By systematically varying the weight fraction of the hydrophilic elastin-like polypeptide (ELP) block and molecular weight of the diblock polypeptide, we designed micelles of different morphologies that modulate the binding avidity of the human wild-type 10th fibronectin domain (Fn3) as a function of shape. We show that wormlike micelles that present the Fn3 domain have a 1000-fold greater avidity for the αvβ3 receptor compared to the monomer ligand and an avidity that is greater than a clinically relevant antibody that is driven by their multivalency. The amplified avidity of these micelles leads to significantly increased cellular internalization, a feature that may have utility for the intracellular delivery of drugs that are loaded into the core of these micelles.
Keyphrases
- cancer therapy
- drug delivery
- type iii
- drug release
- endothelial cells
- drosophila melanogaster
- wild type
- hyaluronic acid
- pluripotent stem cells
- induced pluripotent stem cells
- machine learning
- physical activity
- binding protein
- cell therapy
- single cell
- stem cells
- weight loss
- deep learning
- small molecule
- bone marrow
- reactive oxygen species
- mass spectrometry
- protein protein
- molecularly imprinted
- solid phase extraction