Influence of glycan structure on the colonization of Streptococcus pneumoniae on human respiratory epithelial cells.
Ye-Yu ChunKai Sen TanLisa YuMichelle PangMing Hui Millie WongRei NakamotoWan-Zhen ChuaAmanda Huee-Ping WongZhe Zhang Ryan LewHsiao Hui OngVincent Tak Kwong ChowThai TranDe Yun WangLok-To ShamPublished in: Proceedings of the National Academy of Sciences of the United States of America (2023)
Virtually all living cells are encased in glycans. They perform key cellular functions such as immunomodulation and cell-cell recognition. Yet, how their composition and configuration affect their functions remains enigmatic. Here, we constructed isogenic capsule-switch mutants harboring 84 types of capsular polysaccharides (CPSs) in Streptococcus pneumoniae . This collection enables us to systematically measure the affinity of structurally related CPSs to primary human nasal and bronchial epithelial cells. Contrary to the paradigm, the surface charge does not appreciably affect epithelial cell binding. Factors that affect adhesion to respiratory cells include the number of rhamnose residues and the presence of human-like glycomotifs in CPS. Besides, pneumococcal colonization stimulated the production of interleukin 6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), and monocyte chemoattractantprotein-1 (MCP-1) in nasal epithelial cells, which also appears to be dependent on the serotype. Together, our results reveal glycomotifs of surface polysaccharides that are likely to be important for colonization and survival in the human airway.
Keyphrases
- endothelial cells
- induced pluripotent stem cells
- single cell
- pluripotent stem cells
- stem cells
- escherichia coli
- gene expression
- staphylococcus aureus
- dendritic cells
- cell proliferation
- cystic fibrosis
- binding protein
- mesenchymal stem cells
- peripheral blood
- oxidative stress
- zika virus
- transcription factor
- bone marrow
- cell cycle arrest
- aedes aegypti
- cerebrospinal fluid