Interferon-Inducible Guanylate-Binding Protein 5 Inhibits Replication of Multiple Viruses by Binding to the Oligosaccharyltransferase Complex and Inhibiting Glycoprotein Maturation.
Shaobo WangWanyu LiLingling WangShashi Kant TiwariWilliam BrayLujing WuNa LiHui HuiAlex E ClarkQiong ZhangLingzhi ZhangAaron F CarlinTariq M RanaPublished in: bioRxiv : the preprint server for biology (2024)
Viral infection induces production of type I interferons and expression of interferon-stimulated genes (ISGs) that play key roles in inhibiting viral infection. We found that the interferon-stimulated gene GBP5 is induced by SARS-CoV-2 infection in vitro and in vivo. GBP5 inhibits N-glycosylation of key proteins in multiple viruses, including SARS-CoV-2. Importantly, pharmacological inhibition of Oligosaccharyltransferase (OST) Complex blocks host cell infection by SARS-CoV-2, variants of concern, HIV-1, and IAV, indicating future translational application of our findings.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- binding protein
- dendritic cells
- copy number
- signaling pathway
- genome wide
- poor prognosis
- antiretroviral therapy
- hiv positive
- genome wide identification
- single cell
- human immunodeficiency virus
- hepatitis c virus
- hiv aids
- cell therapy
- immune response
- current status
- gene expression
- dna methylation
- bone marrow
- south africa