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Stem lodging Resistance-1 controls stem strength by positively regulating the biosynthesis of cell wall components in Capsicum annuum L.

Qing LiCanfang FuBozhi YangHuiyang YuHuan HeQing XuWu MiaoRongyun LiuWenchao ChenZhuqing ZhangXuexiao ZouBowen HuLijun Ou
Published in: Horticulture research (2024)
Lodging presents a significant challenge in cultivating high-yield crops with extensive above-ground biomass, yet the molecular mechanisms underlying this phenomenon in the Solanaceae family remain largely unexplored. In this study, we identified a gene, CaSLR1 ( Capsicum annuum Stem Lodging Resistance 1 ), which encodes a MYELOBLASTOSIS (MYB) family transcription factor, from a lodging-affected C. annuum EMS mutant. The suppression of CaSLR1 expression in pepper led to notable stem lodging, reduced thickness of the secondary cell wall, and decreased stem strength. A similar phenotype was observed in tomato with the knockdown of SlMYB61 , the orthologous gene to CaSLR1 . Further investigations demonstrated that CaNAC6 , a gene involved in secondary cell wall (SCW) formation, is co-expressed with CaSLR1 and acts as a positive regulator of its expression, as confirmed through yeast one-hybrid, dual-luciferase reporter assays, and electrophoretic mobility shift assays. These findings elucidate the Ca NAC6- Ca SLR1 module that contributes to lodging resistance, emphasizing the critical role of CaSLR1 in the lodging resistance regulatory network.
Keyphrases
  • cell wall
  • transcription factor
  • genome wide identification
  • poor prognosis
  • copy number
  • genome wide
  • dna binding
  • high throughput
  • optical coherence tomography
  • binding protein