CRISPR/Cas9-mediated knockout of NlCYP6CS1 gene reveals its role in detoxification of insecticides in Nilaparvata lugens (Hemiptera: Delphacidae).

Yan-Chao ZhangYang GaoWen-Nan YeYu-Xuan PengKun Yan Zhu Cong-Fen Gao

Published in: Pest management science (2023)

Our study demonstrates the effect of functional deletion of NlCYP6CS1 on multiple insecticide resistance in N. lugens. For the first time, we applied CRISPR/Cas9 system to reveal the mechanism of insecticide resistance in N. lugens, which may shed light on similar studies in other hemipteran insects. © 2023 Society of Chemical Industry.

Keyphrases

crispr cas
genome editing
aedes aegypti
genome wide
zika virus
copy number
single cell
dna methylation
gene expression
transcription factor
genome wide identification