Single-cell RNA sequencing of adult mouse testes.
Soeren LukassenElisabeth BoschArif Bülent EkiciAndreas WinterpachtPublished in: Scientific data (2018)
Spermatogenesis is an efficient and complex system of continuous cell differentiation. Previous studies investigating the transcriptomes of different cell populations in the testis relied either on sorting cells, cell depletion, or juvenile animals where not all stages of spermatogenesis have been completed. We present single-cell RNA sequencing (scRNA-Seq) data of 2,500 cells from the testes of two 8-week-old C57Bl/6J mice. Our dataset includes all spermatogenic stages from preleptotene to condensing spermatids as well as individual spermatogonia, Sertoli and Leydig cells. The data capture the full continuity of the meiotic and postmeiotic stages of spermatogenesis, and is thus ideally suited for marker discovery, network inference and similar analyses for which temporal ordering of differentiation processes can be exploited. Furthermore, it can serve as a reference for future studies involving single-cell RNA-Seq in mice where spermatogenesis is perturbed.
Keyphrases
- single cell
- rna seq
- high throughput
- induced apoptosis
- cell cycle arrest
- electronic health record
- small molecule
- endoplasmic reticulum stress
- high fat diet induced
- signaling pathway
- gene expression
- type diabetes
- randomized controlled trial
- cell proliferation
- metabolic syndrome
- dna methylation
- machine learning
- current status
- insulin resistance
- wild type
- study protocol
- deep learning