Login / Signup

Measuring the composition of the tumor microenvironment with transcriptome analysis: past, present and future.

Han ZhangXinghua LuBinfeng LuGiuseppe GulloLujia Chen
Published in: Future oncology (London, England) (2024)
Interactions between tumor cells and immune cells in the tumor microenvironment (TME) play a vital role the mechanisms of immune evasion, by which cancer cells escape immune elimination. Thus, the characterization and quantification of different components in the TME is a hot topic in molecular biology and drug discovery. Since the development of transcriptome sequencing in bulk tissue, single cells and spatial dimensions, there are increasing methods emerging to deconvolute and subtype the TME. This review discusses and compares such computational strategies and downstream subtyping analyses. Integrative analyses of the transcriptome with other data, such as epigenetics and T-cell receptor sequencing, are needed to obtain comprehensive knowledge of the dynamic TME.
Keyphrases
  • single cell
  • drug discovery
  • rna seq
  • induced apoptosis
  • gene expression
  • genome wide
  • healthcare
  • cell cycle arrest
  • oxidative stress
  • big data
  • dna methylation
  • cell death
  • binding protein
  • cell proliferation