Adventitious Shoot Regeneration from Leaf Explants in Sinningia Hybrida 'Isa's Murmur'.

As a valuable ornamental plant, Sinningia hybrida 'Isa's Murmur' ( S. hybrida ) has genetic flower diversity, which has great potential to develop different flower characters in the horticultural market. The present study focuses on establishing a practical approach for the sustainable propagation of S. hybrida . Compared with aseptic seeding leaves explants, field-grown leaves explants are more suitable for adventitious shoot regeneration. Adding 0.1 mg L -1 NAA and 2.0 mg L -1 TDZ could obtain the highest adventitious shoot proliferation coefficient (24.5), and the induction rate was 91.7%. The shoot proliferation coefficient (20.7) and the greatest shoot length and induction rate (95.3%) were achieved in 0.1 mg L -1 NAA and 2.0 mg L -1 BA medium, accompanied by rooting formation. Adding 0.5 mg L -1 GA 3 , 1.0 mg L -1 BA, and 0.2 mg L -1 IBA to MS medium can effectively prolong the regenerated buds for rooting. The best for rooting was 1/2 MS medium containing 0.3 mg L -1 IBA, with the maximum number of roots (13.4 per shoot) and survival rate for transplanting (100%). This work aims to build an efficient, definitive, and scalable protocol for S. hybrida regeneration useful for large-scale cultivation and even more protoplast fusion and genetic transformation to develop more colorful or fragrant flowers.