QCM-based immunosensor for rapid detection of Salmonella Typhimurium in food.
Andrea FulgioneMartina CimafonteBartolomeo Della VenturaMarco IannacconeConcetta AmbrosinoFederico CapuanoYolande Thérèse Rose ProrogaRaffaele VelottaRosanna CapparelliPublished in: Scientific reports (2018)
Salmonella Typhimurium is one of the main causes of outbreaks and sporadic cases of human gastroenteritis. At present, the rapid detection of this pathogen is a major goal of biosensing technology applied to food safety. In fact, ISO standardized culture method takes up to ten days to provide a reliable response. In this paper, we describe a relatively simple protocol for detecting Salmonella Typhimurium in chicken meat based on a Quartz-Crystal Microbalance (QCM), which leads to a limit of detection (LOD) less than of 10° CFU/mL and requires a pre-enrichment step lasting only 2 h at 37 °C. The reliability of the proposed immunosensor has been demonstrated through the validation of the experimental results with ISO standardized culture method. The cost-effectiveness of the procedure and the rapidity of the QCM-based biosensor in providing the qualitative response make the analytical method described here suitable for applications in food inspection laboratory and throughout the chain production of food industry.
Keyphrases
- listeria monocytogenes
- label free
- escherichia coli
- sensitive detection
- human health
- endothelial cells
- randomized controlled trial
- systematic review
- risk assessment
- late onset
- gold nanoparticles
- climate change
- mass spectrometry
- early onset
- induced pluripotent stem cells
- liquid chromatography
- real time pcr
- amyotrophic lateral sclerosis