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Emodin-8- O -Glucoside-Isolation and the Screening of the Anticancer Potential against the Nervous System Tumors.

Estera OkonMaryna KovalAnna WawruszakAdrianna Slawinska-BrychKatarzyna SmolinskaMyroslav SheveraAndrzej StepulakWirginia Kukuła-Koch
Published in: Molecules (Basel, Switzerland) (2023)
Emodin-8- O -glucoside (E-8- O -G) is a glycosylated derivative of emodin that exhibits numerous biological activities, including immunomodulatory, anti-inflammatory, antioxidant, hepatoprotective, or anticancer activities. However, there are no reports on the activity of E-8- O -G against cancers of the nervous system. Therefore, the aim of the study was to investigate the antiproliferative and cytotoxic effect of E-8- O -G in the SK-N-AS neuroblastoma, T98G human glioblastoma, and C6 mouse glioblastoma cancer cells. As a source of E-8- O -G the methanolic extract from the aerial parts of Reynoutria japonica Houtt. (Polygonaceae) was used. Thanks to the application of centrifugal partition chromatography (CPC) operated in the descending mode using a mixture of petroleum ether:ethyl acetate:methanol:water (4:5:4:5 v / v / v / v ) and a subsequent purification with preparative HPLC, E-8- O -G was obtained in high purity in a sufficient quantity for the bioactivity tests. Assessment of the cancer cell viability and proliferation were performed with the MTT (3-(bromide 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium), CTG (CellTiter-Glo ® ) and BrdU (5-bromo-2'-deoxyuridine) assays, respectively. E-8- O -G inhibits the viability and proliferation of SK-N-AS neuroblastoma, T98G human glioblastoma multiforme, and C6 mouse glioblastoma cells dose-dependently. E-8- O -G seems to be a promising natural antitumor compound in the therapy of nervous system tumors.
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