Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity.

Hongxin Huang Guanjie Huang Zhihong Tan Yong-Fei Hu Lin Shan Jia-jian Zhou Xin Zhang Shufeng Ma Weiqi Lv Tao Huang Yuchen Liu Liqiang Wang Xiao-Yang Zhao Ying Lin Zhili Rong

Published in: BMC biology (2022)

Our discoveries provide alternative AsCas12a and LbCas12a variants with high specificity and activity, which expand the gene-editing toolbox and can be more suitable for clinical applications.

Keyphrases

crispr cas
genome editing
copy number
structural basis