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Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from 'mam nem', a fermented fish sauce.

Do Thi Bich ThuyAnTien NguyenKuan Shiong KhooKit Wayne ChewMargo CnockaertPeter VandammeYeek-Chia HoNguyen Duc HuyHeriberto Hernández CocoletziPau-Loke Show
Published in: Bioengineered (2021)
This study was aimed to identify and optimize the culture conditions for gamma-aminobutyric acid (GABA) production by a lactic acid bacterium strain isolated from mam nem, a fermented fish sauce. Among the six isolates obtained from mam nem, the MN12 had the most potent GABA-producing capability. The strain was then identified to be Pedioccocus pentosaceus by employing MALDI-TOF-MS and phenylalanyl-tRNA synthase sequencing methods. The initial cell density of 5.106 CFU/mL, monosodium glutamate concentration of 60 mM, initial pH of 7, temperature of 45°C and cultivation time of 72 h were found to be the optimal culture conditions for highest production of GABA, reaching 27.9 ± 0.42 mM, by this strain. The cultivation conditions for GABA production by P. pentosaceus MN12 have been successfully optimized, providing a foundation for the development of fermented foods enriched with GABA.
Keyphrases
  • lactic acid
  • single cell
  • room temperature
  • mass spectrometry
  • cell therapy
  • metal organic framework
  • bone marrow