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Relative Nuclease Resistance of a DNA Aptamer Covalently Conjugated to a Target Protein.

Yudai TabuchiJay YangMasumi Taki
Published in: International journal of molecular sciences (2022)
A major obstacle to the therapeutic application of an aptamer is its susceptibility to nuclease digestion. Here, we confirmed the acquisition of relative nuclease resistance of a DNA-type thrombin binding aptamer with a warhead (TBA 3 ) by covalent binding to a target protein in the presence of serum/various nucleases. When the thrombin-inhibitory activity of TBA 3 on thrombin was reversed by the addition of the complementary strand, the aptamer was instantly degraded by the nucleases, showing that the properly folded/bound aptamer conferred the resistance. Covalently binding aptamers possessing both a prolonged drug effect and relative nuclease resistance would be beneficial for in vivo translational applications.
Keyphrases
  • gold nanoparticles
  • dna binding
  • sensitive detection
  • magnetic nanoparticles
  • label free
  • binding protein
  • genome editing
  • transcription factor
  • protein protein
  • crispr cas
  • quantum dots
  • photodynamic therapy
  • drug induced