cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries.
Meifang QiUtthara NayarLeif S LudwigNikhil WagleEsther RheinbayPublished in: BMC bioinformatics (2021)
cDNA-detector is a user-friendly and accurate tool to detect and remove cDNA detection in NGS libraries. This two-step design reduces the risk of true variant removal since it allows for manual review of candidates. We find that contamination with intentionally and accidentally introduced cDNAs is an underappreciated problem even in widely-used consortium datasets, where it can lead to spurious results. Our findings highlight the importance of sensitive detection and removal of contaminant cDNA from NGS libraries before downstream analysis.
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- risk assessment
- drinking water
- health risk
- quantum dots
- label free
- real time pcr
- high resolution
- single molecule
- human health
- magnetic resonance
- single cell
- magnetic resonance imaging
- mass spectrometry
- climate change
- heavy metals
- data analysis
- circulating tumor cells