Water Extract of Lysimachia christinae Inhibits Trabecular Bone Loss and Fat Accumulation in Ovariectomized Mice.
Ki-Shuk ShimYoun-Hwan HwangSeon-A JangTaesoo KimHyunil HaPublished in: Nutrients (2020)
In Asia, extracts of Lysimachia christinae have been used for liver or urinogenital system-related diseases in traditional medicine. In this study, we investigated the effects of the water extract of L. christinae (WELC) on receptor activator of nuclear factor-kappa Β ligand (RANKL)-induced osteoclastic differentiation of bone marrow macrophages, and on osteoporosis and obesity in ovariectomy mice. RANK signaling pathways related to osteoclast differentiation were examined by real-time polymerase chain reaction (PCR) and western blot analysis. Additionally, we performed micro-computed tomography to assess trabecular bone loss, histological analysis for fat accumulation in adipose, liver, and bone tissues, and phytochemical profiling for WELC characterization. WELC significantly inhibited osteoclast differentiation by downregulating RANKL-induced mitogen-activated protein kinase (MAPK)/c-Fos/nuclear factor of activated T-cells (NFAT) signaling in osteoclast precursors and ovariectomy-induced trabecular loss by suppressing osteolcastic bone resorption. WELC markedly decreased ovariectomy-induced body weight gain and fat accumulation in adipose, liver, and bone tissues. Furthermore, ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) identified 16 phytochemicals in WELC when compared with the mass fragmentation of standard chemicals. Collectively, these results suggest that WELC might possess beneficial effects on postmenopausal osteoporosis by inhibiting osteoclast differentiation and obesity by suppressing fat accumulation.
Keyphrases
- bone loss
- nuclear factor
- bone mineral density
- weight gain
- ms ms
- toll like receptor
- adipose tissue
- liquid chromatography tandem mass spectrometry
- insulin resistance
- high glucose
- high fat diet induced
- signaling pathway
- computed tomography
- bone marrow
- postmenopausal women
- metabolic syndrome
- weight loss
- gene expression
- drug induced
- oxidative stress
- body mass index
- magnetic resonance imaging
- simultaneous determination
- fatty acid
- magnetic resonance
- body composition
- immune response
- south africa
- anti inflammatory
- epithelial mesenchymal transition
- endoplasmic reticulum stress
- pi k akt
- endothelial cells
- positron emission tomography
- pet ct
- stress induced
- cell proliferation
- protein kinase
- atomic force microscopy