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Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli.

Akiko OnoJili ZhangYuki TanakaChihiro SatoAkiyoshi YodaMakoto OgataTakahito NomuraTomohiro Suzuki
Published in: Bioscience, biotechnology, and biochemistry (2023)
Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.
Keyphrases
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  • life cycle
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  • gene expression
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