The human mast cell line-1 cell membrane chromatography coupled with HPLC-ESI-MS/MS method for screening potentical anaphylactic components from chuanxinlian injection.
Yuanyuan LinCheng WangYajing HouHuaizhen HeLimin HuangLiu YangLangchong HePublished in: Biomedical chromatography : BMC (2017)
Chuanxinlian injection is a traditional Chinese medicine injection widely used in China to treat sore throat, cough and dysentery, although a high occurrence of severe adverse reactions has been reported in clinical practice in recent years. In the present study, a human mast cell line-1 cell membrane chromatography coupled with HPLC-ESI-MS/MS method was established to screen and identify potentical anaphylactic components in chuanxinlian injection, and the dehydroandrographolide was identified as a potential anaphylactic component. In vitro anaphylactic assay showed that intracellular Ca2+ concentration clearly increased under dehydroandrographolide (100 μm) treatment. β-Hexosaminidase and histamine release in human mast cell line-1 cells were both markedly enhanced with increased concentrations of dehydroandrographolide, confirming the anaphylactic activity of dehydroandrographolide. The application for chuanxinlian injection in this study suggested that the developed human mast cell line-1 cell membrane chromatography coupled with HPLC-ESI-MS/MS system may be effective and rapid for screening the potentical anaphylactic components from complex samples.
Keyphrases
- ms ms
- endothelial cells
- high performance liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- induced pluripotent stem cells
- pluripotent stem cells
- clinical practice
- simultaneous determination
- ultra high performance liquid chromatography
- risk assessment
- high speed
- high throughput
- emergency department
- climate change
- induced apoptosis
- quantum dots
- oxidative stress
- solid phase extraction
- human health
- protein kinase
- adverse drug