Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes.
Zhi-Yu ChenShih-Heng ChenChih-Hao ChenPang-Yung ChouChun-Chen YangFeng-Huei LinPublished in: Polymers (2020)
Tendon healing after injury is relatively slow, mainly because of the weak activity and metabolic properties of tendon cells (tenocytes). Bletilla striata polysaccharide (BSP) has been reported to enhance cell proliferation. Here, we aimed to increase tendon cell proliferation by BSP treatment. We isolated tenocytes from the flexor tendon of human origin. Moreover, we improved the process of extracting BSP. When human tenocytes (HTs) were treated with 100 μg/mL BSP, the MEK/ERK1/2 and PI3K/Akt signaling pathways were activated, thereby enhancing the proliferation ability of tenocytes. BSP treatment also increased the migration of HTs and their ability to secrete the extracellular matrix (Col-I and Col-III). In conclusion, BSP was successfully extracted from a natural Chinese herbal extract and was shown to enhance tenocytes proliferation, migration and collagen release ability. This study is the first to demonstrate improved healing of tendons using BSP.
Keyphrases
- pi k akt
- signaling pathway
- cell proliferation
- cell cycle arrest
- endothelial cells
- induced apoptosis
- extracellular matrix
- induced pluripotent stem cells
- anterior cruciate ligament reconstruction
- pluripotent stem cells
- cell cycle
- epithelial mesenchymal transition
- rotator cuff
- oxidative stress
- cell death
- water soluble
- tissue engineering