The MIR34B/C genomic region contains multiple potential regulators of multiciliogenesis.
Amélie CavardElisa RedmanOlivier MerceySophie AbelanetMagali PlaisantMarie-Jeanne ArguelVirginie MagnoneSandra Ruiz GarcíaGéraldine RiosMarie DeprezKévin LebrigandGilles PonzioIgnacio CaballeroPascal BarbryLaure-Emmanuelle ZaragosiBrice MarcetPublished in: FEBS letters (2023)
The MIR449 genomic locus encompasses several regulators of multiciliated cell formation (multiciliogenesis). The miR-449 homologues miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse or pig multiciliogenesis models. BTG4, LAYN and HOATZ transcripts were expressed in both precursors and mature multiciliated cells. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.
Keyphrases
- single cell
- rna seq
- high throughput
- long non coding rna
- cell proliferation
- genome wide association study
- endothelial cells
- transcription factor
- long noncoding rna
- poor prognosis
- induced apoptosis
- binding protein
- copy number
- high resolution
- amino acid
- mesenchymal stem cells
- big data
- machine learning
- mass spectrometry
- optical coherence tomography
- human health
- induced pluripotent stem cells
- bone marrow
- signaling pathway
- high speed
- label free
- data analysis
- risk assessment
- deep learning