High-content analysis of proteostasis capacity in cellular models of amyotrophic lateral sclerosis (ALS).
Isabella A Lambert-SmithVictoria K ShephardLuke McAlaryJustin J YerburyDarren N SaundersPublished in: Scientific reports (2024)
Disrupted proteome homeostasis (proteostasis) in amyotrophic lateral sclerosis (ALS) has been a major focus of research in the past two decades. However, the proteostasis processes that become disturbed in ALS are not fully understood. Obtaining more detailed knowledge of proteostasis disruption in association with different ALS-causing mutations will improve our understanding of ALS pathophysiology and may identify novel therapeutic targets and strategies for ALS patients. Here we describe the development and use of a novel high-content analysis (HCA) assay to investigate proteostasis disturbances caused by the expression of several ALS-causing gene variants. This assay involves the use of conformationally-destabilised mutants of firefly luciferase (Fluc) to examine protein folding/re-folding capacity in NSC-34 cells expressing ALS-associated mutations in the genes encoding superoxide dismutase-1 (SOD1 A4V ) and cyclin F (CCNF S621G ). We demonstrate that these Fluc isoforms can be used in high-throughput format to report on reductions in the activity of the chaperone network that result from the expression of SOD1 A4V , providing multiplexed information at single-cell resolution. In addition to SOD1 A4V and CCNF S621G , NSC-34 models of ALS-associated TDP-43, FUS, UBQLN2, OPTN, VCP and VAPB mutants were generated that could be screened using this assay in future work. For ALS-associated mutant proteins that do cause reductions in protein quality control capacity, such as SOD1 A4V , this assay has potential to be applied in drug screening studies to identify candidate compounds that can ameliorate this deficiency.
Keyphrases
- amyotrophic lateral sclerosis
- high throughput
- single cell
- quality control
- poor prognosis
- binding protein
- healthcare
- end stage renal disease
- single molecule
- emergency department
- molecular dynamics simulations
- ejection fraction
- genome wide
- newly diagnosed
- nitric oxide
- chronic kidney disease
- dna methylation
- prognostic factors
- transcription factor
- resting state
- cell cycle
- heat stress
- smoking cessation