Investigation of Physical Characteristics and In Vitro Anti-Inflammatory Effects of Fucoidan from Padina arborescens : A Comprehensive Assessment against Lipopolysaccharide-Induced Inflammation.
Hyo-Geun LeeN M LiyanageFengqi YangYoung-Sang KimSeung-Hong LeeSeok-Chun KoHye-Won YangYung Hyun ChoiPublished in: Marine drugs (2024)
A biocompatible, heterogeneous, fucose-rich, sulfated polysaccharide (fucoidan) is biosynthesized in brown seaweed. In this study, fucoidan was isolated from Padina arborescens (PAC) using celluclast-assisted extraction, purified, and evaluated for its anti-inflammatory potential in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Structural analyses were performed using Fourier transform infrared (FTIR) and scanning electron microscopy. Among the purified fucoidans, fucoidan fraction 5 (F5) exhibited strong inhibitory activity against LPS-induced nitric oxide (NO) production and pro-inflammatory cytokine generation through the regulation of iNOS/COX-2, MAPK, and NF-κB signaling in LPS-induced RAW 264.7 cells. Determination of the structural characteristics indicated that purified F5 exhibited characteristics similar to those of commercial fucoidan. In addition, further analyses suggested that F5 inhibits LPS-induced toxicity, cell death, and NO generation in zebrafish models. Taken together, these findings imply that P. arborescens fucoidans have exceptional anti-inflammatory action, both in vitro and in vivo, and that they may have prospective uses in the functional food sector.
Keyphrases
- lps induced
- inflammatory response
- lipopolysaccharide induced
- cell cycle arrest
- induced apoptosis
- cell death
- anti inflammatory
- electron microscopy
- oxidative stress
- nitric oxide
- toll like receptor
- signaling pathway
- pi k akt
- endoplasmic reticulum stress
- physical activity
- mental health
- nitric oxide synthase
- risk assessment
- climate change
- hydrogen peroxide
- ionic liquid
- drug release
- nuclear factor