Intense light as anticoagulant therapy in humans.
Yoshimasa OyamaSydney ShuffPavel Davizon-CastilloNathan ClendenenTobias EcklePublished in: PloS one (2020)
Blood coagulation is central to myocardial ischemia and reperfusion (IR) injury. Studies on the light elicited circadian rhythm protein Period 2 (PER2) using whole body Per2-/- mice found deficient platelet function and reduced clotting which would be expected to protect from myocardial IR-injury. In contrast, intense light induction of PER2 protected from myocardial IR-injury while Per2 deficiency was detrimental. Based on these conflicting data, we sought to evaluate the role of platelet specific PER2 in coagulation and myocardial ischemia and reperfusion injury. We demonstrated that platelets from mice with tissue-specific deletion of Per2 in the megakaryocyte lineage (Per2loxP/loxP-PF4-CRE) significantly clot faster than platelets from control mice. We further found increases in infarct sizes or plasma troponin levels in Per2loxP/loxP-PF4-CRE mice when compared to controls. As intense light increases PER2 protein in human tissues, we also performed translational studies and tested the effects of intense light therapy on coagulation in healthy human subjects. Our human studies revealed that intense light therapy repressed procoagulant pathways in human plasma samples and significantly reduced the clot rate. Based on these results we conclude that intense light elicited PER2 has an inhibitory function on platelet aggregation in mice. Further, we suggest intense light as a novel therapy to prevent or treat clotting in a clinical setting.
Keyphrases
- endothelial cells
- high fat diet induced
- left ventricular
- acute myocardial infarction
- gene expression
- atrial fibrillation
- magnetic resonance
- stem cells
- metabolic syndrome
- induced pluripotent stem cells
- machine learning
- mesenchymal stem cells
- coronary artery disease
- cell therapy
- artificial intelligence
- pluripotent stem cells
- big data
- acute ischemic stroke
- heart rate
- skeletal muscle
- contrast enhanced