Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine.
Gyu Seong YeomIn-Ho SongShrikant Dashrath WarkadPramod B ShindeTaewoon KimSeong-Min ParkSatish Balasaheb NimsePublished in: Biosensors (2021)
The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (ABIA) along with the design and development of a portable fluorescence spectrometer device (CysDDev) for detecting cysteine in simulated human urine. ABIA showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed CysDDev. Further, ABIA also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- endothelial cells
- loop mediated isothermal amplification
- dna damage
- high resolution
- induced pluripotent stem cells
- molecular docking
- amino acid
- induced apoptosis
- pluripotent stem cells
- papillary thyroid
- signaling pathway
- reactive oxygen species
- cell cycle arrest
- cell death
- fluorescence imaging
- energy transfer
- cell proliferation