SDF2L1 Inhibits Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma.
Liqian ZhangZunni ZhangLiuqun QinXiang ShiQi-Sheng SuWuning MoPublished in: BioMed research international (2020)
The purpose of this study was to explore the relationship between stromal cell-derived factor 2-like 1 (SDF2L1) and nasopharyngeal carcinoma (NPC). 12 NPC tissues and 12 chronic nasopharyngitis tissues were involved in our study. Quantitative real-time PCR (qRT-PCR) and Western Blot were utilized to detect the expression of SDF2L1. Besides, immunofluorescence analysis was utilized to determine the protein expression of 97 paraffin-embedded NPC tissues and 58 nasopharyngitis tissues. Biological functional experiment included Cell Counting Kit-8 (CCK-8) assay, cell clone formation assay, cell scratch migration assay, Transwell migration assay, and Transwell invasion assay. All data were analyzed by SPSS. Results showed that downexpression of SDF2L1 was prominently present in NPC tissues and cells. Furthermore, silencing the expression of SDF2L1 promoted NPC proliferation, migration, and invasion in vitro, while overexpression of SDF2L1 has the opposite effect. In conclusion, SDF2L1 may act as a cancer suppressor gene, play a crucial role in the occurrence and development of NPC, and be a new therapeutic target or prognostic indicator for NPC.
Keyphrases
- high throughput
- gene expression
- cell proliferation
- single cell
- poor prognosis
- cell therapy
- real time pcr
- induced apoptosis
- risk assessment
- cell cycle
- long non coding rna
- machine learning
- oxidative stress
- high resolution
- south africa
- dna methylation
- young adults
- cell cycle arrest
- mass spectrometry
- artificial intelligence
- data analysis