Affordable Method for Hematocrit Determination in Murine Models.
Siobhan BranfieldA Valance WashingtonBarbara ManfrediPublished in: Current protocols (2024)
Hematocrit (Hct) is a powerful tool often used in a clinical setting for the diagnosis of blood conditions such as anemia. It is also used in the research field as a hematological parameter in both human and mouse models. Measuring Hct, however, involves the use of expensive standardized equipment (such as a CritSpin™ Microhematocrit Centrifuge). Here, we describe a novel, simple, and affordable method to determine the Hct in untreated wild-type (WT) mice and phenylhydrazine (PHZ)-induced anemic mice with reasonable accuracy, using a benchtop centrifuge commonly available in laboratories. Hct of murine samples processed with a benchtop centrifuge, when compared to the standardized method CritSpin™, showed comparable results. This approach for determining Hct of murine can prove useful to research laboratories that cannot afford specialized equipment for Hct studies. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Affordable Method for Hematocrit Determination in Murine Models Basic Protocol 2: Murine Sample Validation Support Protocol: Phenylhydrazine-induced anemia in wild-type (WT) mice.
Keyphrases
- wild type
- cell cycle arrest
- randomized controlled trial
- high glucose
- endothelial cells
- cell death
- diabetic rats
- high fat diet induced
- chronic kidney disease
- palliative care
- metabolic syndrome
- drug induced
- solid phase extraction
- molecularly imprinted
- cell proliferation
- adipose tissue
- skeletal muscle
- mass spectrometry
- induced pluripotent stem cells