An endochondral ossification approach to early stage bone repair: Use of tissue-engineered hypertrophic cartilage constructs as primordial templates for weight-bearing bone repair.
Amos MatsikoEmmet M ThompsonCai Lloyd-GriffithGráinne M CunniffeTatiana VinardellJohn P GleesonDaniel John KellyFergal Joseph O'BrienPublished in: Journal of tissue engineering and regenerative medicine (2018)
Mimicking endochondral ossification to engineer constructs offers a novel solution to overcoming the problems associated with poor vascularisation in bone repair. This can be achieved by harnessing the angiogenic potency of hypertrophic cartilage. In this study, we demonstrate that tissue-engineered hypertrophically primed cartilage constructs can be developed from collagen-based scaffolds cultured with mesenchymal stem cells. These constructs were subsequently implanted into femoral defects in rats. It was evident that the constructs could support enhanced early stage healing at 4 weeks of these weight-bearing femoral bone defects compared to untreated defects. This study demonstrates the value of combining knowledge of development biology and tissue engineering in a developmental engineering inspired approach to tissue repair.
Keyphrases
- early stage
- tissue engineering
- bone mineral density
- mesenchymal stem cells
- soft tissue
- body mass index
- bone loss
- extracellular matrix
- physical activity
- healthcare
- weight loss
- bone regeneration
- bone marrow
- weight gain
- postmenopausal women
- body composition
- squamous cell carcinoma
- mental health
- machine learning
- sentinel lymph node
- gestational age