Precision 1070 nm Ultrafast Laser-Induced Photothrombosis of Depth-Targeted Vessels In Vivo.
Liang ZhuMengqi WangPeng FuYin LiuHequn ZhangAnna Wang RoeWang XiPublished in: Small methods (2022)
The cerebrovasculature plays an essential role in neurovascular and homeostatic functions in health and disease conditions. Many efforts have been made for developing vascular thrombosis methods to study vascular dysfunction in vivo, while technical challenges remain, such as accuracy and depth-selectivity to target a single vessel in the cerebral cortex. Herein, this paper first demonstrates the evaluation and quantification of the feasibility and effects of Rose Bengal (RB)-induced photothrombosis with 720-1070 nm ultrafast lasers in a raster scan. A flexible and reproducible approach is then proposed to employ a 1070 nm ultrafast laser with a spiral scan for producing RB-induced occlusion, which is described as precision ultrafast laser-induced photothrombosis (PLP). Combine with two-photon microscopy imaging, this PLP displays highly precise and fast occlusion induction of various vessel types, sizes, and depths, which enhances the precision and power of the photothrombosis protocol. Overall, the PLP method provides a real-time, practical, precise, and depth-selected single-vessel photothrombosis technology in the cerebral cortex with commercially available optical equipment, which is crucial for exploring brain vascular function with high spatial-temporal resolution in the brain.
Keyphrases
- optical coherence tomography
- high resolution
- functional connectivity
- resting state
- energy transfer
- photodynamic therapy
- high glucose
- diabetic rats
- cerebral ischemia
- computed tomography
- high speed
- subarachnoid hemorrhage
- white matter
- public health
- healthcare
- electron transfer
- single molecule
- randomized controlled trial
- oxidative stress
- mental health
- drug induced
- risk assessment
- cancer therapy
- multiple sclerosis
- blood brain barrier
- light emitting
- health information
- contrast enhanced
- cerebral blood flow