Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media.
Francesco AgostiniFrancesca Maria RossiDonatella AldinucciMonica BattistonElisabetta LombardiStefania ZanolinSamuele MassarutPier Camillo ParodiAlessandro Da PonteGiovanni TessitoriBarbara PivettaCristina DuranteMario MazzucatoPublished in: Stem cell research & therapy (2018)
This paper reports a GMP-compliant approach for freezing SVF cells isolated from adipose tissue by a standardized protocol. Moreover, an ASC expansion method in controlled culture conditions and without involvement of animal-derived additives was reported.
Keyphrases
- adipose tissue
- stem cells
- induced apoptosis
- insulin resistance
- biofilm formation
- cell cycle arrest
- bone marrow
- randomized controlled trial
- high fat diet
- ionic liquid
- endoplasmic reticulum stress
- emergency department
- oxidative stress
- type diabetes
- pseudomonas aeruginosa
- staphylococcus aureus
- cell therapy
- escherichia coli
- skeletal muscle
- mesenchymal stem cells
- cystic fibrosis
- pi k akt
- drug induced