Antiinfective properties of ursolic acid-loaded chitosan nanoparticles against Staphylococcus aureus.
Fatemeh GhasemzadehGhasem Najafpour DarziMaedeh MohammadiPublished in: Turkish journal of chemistry (2021)
The present study aimed to synthesize ursolic acid-loaded chitosan nanoparticles (UA-Ch-NPs) as an antiinfective agent against 21 Staphylococcus aureus isolates. The UA-Ch-NPs were synthesized by a simple method and then characterized by TEM, FTIR, DLS-zeta potential, and XRD analyses. According to the characterization results, highly dispersed spherical nanoparticles with a mean diameter of 258 nm and a zeta potential of + 40.1 mV were developed. The antibacterial properties of UA-Ch-NPs were investigated and their inhibitory effect on biofilm formation was demonstrated by AFM. Finally, the expression levels of icaA and icaD were measured using real-time PCR. Results indicated that the minimum inhibitory concentration (MIC) of UA and UA-Ch-NPs against S. aureus was 64 and 32 µg/mL, respectively. The treatment of bacterial cells with UA-Ch-NPs significantly decreased the expression of icaA and icaD genes which are engaged in biofilm formation. Our results indicated that UA-Ch-NPs could be a promising material for antibacterial and antibiofilm applications.
Keyphrases
- biofilm formation
- staphylococcus aureus
- room temperature
- pseudomonas aeruginosa
- drug delivery
- oxide nanoparticles
- candida albicans
- wound healing
- escherichia coli
- poor prognosis
- real time pcr
- methicillin resistant staphylococcus aureus
- induced apoptosis
- binding protein
- ionic liquid
- atomic force microscopy
- cancer therapy
- genome wide
- cystic fibrosis
- human health
- cell death
- climate change
- endoplasmic reticulum stress
- walled carbon nanotubes
- mass spectrometry
- genetic diversity