Quantification of circulating microRNAs by droplet digital PCR for cancer detection.

Priscila D R CirilloKatia MargiottiAlvaro MesoracaClaudio Giorlandino

Published in: BMC research notes (2020)

We have described an efficient protocol for an accurate absolute quantification of circulating miRNAs in healthy and ovarian cancer serum samples. Our data showed that ddPCR methodology can accurately measure circulating miRNAs levels and that can be a useful tool in biomarkers discovery for ovarian cancer detection.

Keyphrases

real time pcr
loop mediated isothermal amplification
high throughput
papillary thyroid
randomized controlled trial
label free
small molecule
high resolution
single cell
squamous cell
machine learning
mass spectrometry
deep learning
childhood cancer