Light-assisted small-molecule screening against protein kinases.
Álvaro Inglés-PrietoEva ReichhartMarkus K MuellnerMatthias NowakSebastian M B NijmanMichael GruschHarald JanovjakPublished in: Nature chemical biology (2015)
High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.
Keyphrases
- small molecule
- high throughput
- protein protein
- tyrosine kinase
- drug discovery
- single cell
- epidermal growth factor receptor
- endothelial cells
- binding protein
- amino acid
- high resolution
- induced pluripotent stem cells
- stem cells
- cell therapy
- healthcare
- emergency department
- health information
- gene expression
- mesenchymal stem cells
- bone marrow
- high speed
- mass spectrometry
- pluripotent stem cells